Olympus FV1000 with Multi-Photon


– 3D multi-color confocal imaging/optical sectioning (4 channel detection)

– Two-photon imaging of thick samples (long working distance objectives and a fs pulsed, tunable laser, 690 – 1000 nm)

– Fluorescence Recovery After Photobleaching (FRAP)

– Real time data capture with simultaneous (dual scan units) image-scan for coincident bleach/photo-activation/photo-conversion while imaging

– Fluorescence spectra analysis

– Variable band width detectors for spectral analysis



Regular confocal imaging: BIF members: $24/h, Academic users: $40/h, Industry users: $60/h

Two-photon imaging: BIF members: $39.60/h, Academic users: $66/h, Industry users: $150/h



Olympus BX61Wi upright microscope w/ epifluorescence illumination

Prior motorized stage (programmable sample repositioning or image tiling)


Conventional Confocal Imaging:

Olympus FV1000 confocal scan head with SIM (simultaneous) dual scan head for coincident imaging and photo stimulation/activation/bleaching

Solid-state laser lines for 405, 440, 473, 559 and 635 nm excitation

2 variable band-width tunable detectors, 2 detectors with conventional BP filters for 4 channel fluorescence detection

Forward propagation detector for ‘transmitted light’ image generation


Two-Photon Imaging:

Spectra-Physics MaiTai laser (690 – 1000 nm) with DeepSeedispersion compensation unit

Suitable for blue/green/yellow fluorescence protein imaging and SHG

3 or 4 NDD (non-descanned detector) channel back-propagated signal detection

2 NDD channel forward propagated signal detection


Conventional Objective Lenses:

10x/NA 0.4/Air

20x/NA 0.75/Air

40x/NA 0.95/Air

60x/NA 1.42/Oil


Specialized Objective Lenses (Water Dipping Lenses):

10x/NA 0.6; WD 3mm; dedicated two-photon imaging

25x/NA 1.05; WD 2mm; dedicated two-photon imaging

40x/NA 0.8; WD 3.3mm; compatible for two-photon imaging

60x/NA 1.1; WD 1.5mm; compatible for two-photon imaging



Olympus Fluoview software operated by Windows XP