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Image courtesy of Brian Duarte (Hamelin lab, Forest Conservation Sciences). 3D reconstruction of the confocal images acquired with the Leica Stellaris 8; Cronartium ribicola (causal agent of white pine blister rust) aeciospores on Ribes nigrum (black currant leaves), germinating and entering through the Stomata, 3 days after inoculation. The fungus was stained with Alexa fluor […]
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Robyn Birch (Wang lab, Materials Eng.) used the Leica Stellaris 8 Dive to look at trabecular bone with a mix of woven and lamellar bone (gray) using second harmonic generation (SHG), and blood cells (green) present in the marrow. Autofluorescence of blood cells was acquired using the multi-photon microscopy.
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Image courtesy of Sawyer d’Entremont (Upham lab, CHBE). SEM micrographs of elongated particles of metal with carbon covering surface (left) and elongated particles of metal with carbon covering surface, containing short multi-walled carbon nanotubes (right). Images were acquired with the Zeiss XB350 SEM.
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Dr. Eunji Park, a postdoctoral researcher in the Leander lab (Zoology), was able to visualize the different local microenvironment within a marine organism using the Leica Stellaris 8 FALCON. DNA was stained with a fluorescent dye. The left image shows a fluorescence image based on the intensity; the right image shows a color-coded fluorescence lifetime […]
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Images of heme-dependent enzyme microcrystals acquired with the Olympus BX53 by Nana Mirotadze (Ryan lab, Chemistry). She conducted bright-field (left) and phase contrast (right) imaging.
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TEM micrographs of chitin nanocrystals suspension acquired with the FEI Tecnai Spirit TEM by Andreas Ortiz (MacLachlan lab, Chemistry). Samples were prepared using acid hydrolysis with chitin from shrimp shells.
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Dr. Nicole Coots uses the Leica Stellaris 8 to study copepods collected from the open ocean in Bermuda. She used confocal and Fluorescence LifeTime Imaging Microscopy (FLIM). DAPI staining (cyan), Far-red autofluorescence (magenta), universal eukaryote FISH probe (yellow), a color-coded image of the fluorescence lifetime across the sample.
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Image of the UBQ1pro-GFP-MBD expressed in Arabidopsis seedling. This image was acquired with the Evident SpinSR microscope by Sean Ritter, PhD student in Wasteneys lab (UBC Botany).
FOSI seminar: UBC Imaging Facilities in Focus Sept 25 at 2:30pm LSC3 or Zoom
Focused On Scientific Imaging (FOSI) seminar will take place on September 25th (Thursday) at 2:30 pm in Life Sciences Centre 1003 (LSC3) or Zoom (Hybrid). “UBC Imaging Facilities in Focus” Super Resolution Microscopy Core (LSI Imaging): Guang Gao NeuroImaging and NeuroComputation Centre: Jeff LeDue High-Resolution Macromolecular Cryo-Electron Microscopy: Claire Atkinson Before the talk (2:15-2:30pm), […]
New Leica Stellaris 8 Dive (confocal and multiphoton imaging) -open for training-
A new confocal microscope, Leica Stellaris 8 Dive, has been installed at the BIF! The Leica Stellaris 8 confocal system is capable of multi-color, 3D imaging, tiling and stitching, multi-point imaging, Fluorescence Recovery After Photobleaching (FRAP) with 4 tunable HyD detectors and 4 solid laser lines (405, 488, 561 and 638 nm). The upright microscope […]