Capabilities:
– Imaging and targeting of thin layers of the cell surface (TIRF and variable-angle near TIRF mode)
– Single-molecule analysis
– Fluorescence Recovery After Photobleaching (FRAP) on TIRF
– High-sensitivity, high-speed imaging with sCMOS camera
Cost:
BIF members: $24/h, Academic users: $40/h
Specifications:
Microscope:
Zeiss Observer Z1 inverted microscope
TempModuleS: Temperature-control stage and objective lens heater
CO2/O2ModuleS: CO2 and O2 chamber
Light Source:
Solid-laser lines for 405, 488, 561 and 633 nm
COLIBRI 2 LED lines for 365, 470, and 590 nm
Objectives:
63x/NA 1.46/Oil Korr TIRF
Filter Cube/Dichroic Mirrors:
- 76 Ex: TBP 406+489+561/TFT 427+503+578/Em TBP 459+525+608
- Empty
- 49 Ex: 365/FT395/BP445/50
- 52 Ex: BP488/20/FT505/BP530/50
- 62 Ex: BP370/40, BP478/28, BP585/35/TFT 395+495+610/EM DBP 426/46+527/54+LP615
- 77 Ex: TBP 483+564+642/TFT 506+582+659/Em TBP 526+601+688
Others:
TempModuleS: Temperature-control stage and objective lens heater
CO2/O2ModuleS: CO2 and O2 chamber
Camera and Control:
Hamamatsu Flash 4.0 sCMOS camera, 100 frames per second at 2048 x 2048 pixels with a resolution of 6.5 mm/pixel
Zeiss Zen2 software operated by Windows 7