Zeiss TIRF Microscope

Capabilities:

– Imaging and targeting of thin layers of the cell surface (TIRF and variable-angle near TIRF mode)

– Single-molecule analysis

– Fluorescence Recovery After Photobleaching (FRAP) on TIRF

– High-sensitivity, high-speed imaging with sCMOS camera

 

Cost:

BIF members: $24/h, Academic users: $40/h

Specifications:

Microscope:

Zeiss Observer Z1 inverted microscope

TempModuleS: Temperature-control stage and objective lens heater

CO2/O2ModuleS: CO2 and O2 chamber

 

Light Source:

Solid-laser lines for 405, 488, 561 and 633 nm

COLIBRI 2 LED lines for 365, 470, and 590 nm

 

Objectives:

63x/NA 1.46/Oil Korr TIRF

 

Filter Cube/Dichroic Mirrors:

  1. 76 Ex: TBP 406+489+561/TFT 427+503+578/Em TBP 459+525+608
  2. Empty
  3. 49 Ex: 365/FT395/BP445/50
  4. 52 Ex: BP488/20/FT505/BP530/50
  5. 62 Ex: BP370/40, BP478/28, BP585/35/TFT 395+495+610/EM DBP 426/46+527/54+LP615
  6. 77 Ex: TBP 483+564+642/TFT 506+582+659/Em TBP 526+601+688

 

Others:

TempModuleS: Temperature-control stage and objective lens heater

CO2/O2ModuleS: CO2 and O2 chamber

 

Camera and Control:

Hamamatsu Flash 4.0 sCMOS camera, 100 frames per second at 2048 x 2048 pixels with a resolution of 6.5 mm/pixel

Zeiss Zen2 software operated by Windows 7